Within the Rob2 mission we investigate through ground-based experiments and space flight whether microgravity and cosmic radiation has an impact on the DNA repair processes of living organisms using the bdelloid rotifer Adineta vaga as model system.
During this Rob2 mission A. vaga individuals submitted to desiccation and 500 Gy X-ray or proton radiation (inducing DNA DSBs) will be sent to space. Their capacity to repair their broken genome will be compared in space and on earth. Indeed, desiccated and irradiated A. vagawill be rehydrated respectively at 1G and 0G microgravity on ISS, and on earth.
Survival, fertility and DNA damages at genomic level will be investigated as well as the repair kinetic and gene expression (RNAseq).
The first step is the desiccation of 1.800.000 A. vaga individuals , under controlled conditions, in the LEGE laboratory (UNamur). This was perfomed by technician J. Berthe and postdocs V. Moris and B. Hespeels on November 20th, 2020.